![]() Klebsiella pneumoniae growing on a slant of EMB agar or culture of other capsulated bacteria and non-capsulated bacteria ![]() Test bacteria: 36-48 hour culture of capsulated bacteria e.g.Thus, the capsule appears as a faint blue halo around a purple cell. As the copper sulfate decolorizes the capsule, it also counterstains the capsule. It decolorizes the capsule by washing out the crystal violet, but will not decolorize the cell. A 20% copper sulfate solution serves a dual role as both the decolorizing agent and counterstain. There is no mordant in the capsule staining procedure. ![]() In this type of capsule staining procedure, the primary stain is crystal violet, and all parts of the cell take up the purple crystal violet stain. The capsule (if present) will appear clear against the dark background (capsule does not take any stain).Įxpert opinion: Tasha Sturm, Microbiologist at Cabrillo College, “I use Nigrosin instead of India ink as Nigrosin gives a more even background and spreads little easier”.The bacterial cells will be stained purple (bacterial cells take crystal violet-basic dyes as they are negatively charged).The background will be dark (color of India ink).This method is used for demonstrating Cryptococcus. The capsule is seen as a clear halo around the microorganism against the black background. In this method, two dyes, crystal violet, and India ink are used. Two commonly used methods are discussed here: A. The results (stain of the cells, background, and capsule) depend on the method used. Various types of methods are available for the demonstration of bacterial capsules.
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